Solar-induced mitochondrial mutagenesis and dysfunction in human skin

............................................................................................................................................ 24 Background ....................................................................................................................................... 25 Questions addressed ........................................................................................................................ 25 Experimental design .......................................................................................................................... 25 Results ............................................................................................................................................... 26 Conclusion ......................................................................................................................................... 27 Acknowledgements ........................................................................................................................... 28 References ........................................................................................................................................ 29 Data S 1 – Materials and methods .................................................................................................... 32 Figure S 1 – mtDNA copy number in juvenile skin samples ............................................................. 40 IV. Shortwave UV-induced damage as part of the solar damage spectrum is not a major contributor to mitochondrial dysfunction ................................................................................. 42 Abstract ............................................................................................................................................. 42............................................................................................................................................ 42 Introduction ........................................................................................................................................ 43 Materials and methods ...................................................................................................................... 44 Results ............................................................................................................................................... 47 Discussion ......................................................................................................................................... 52 Acknowledgements ........................................................................................................................... 53